PREPARATION OF CLEANING SOLUTION: ETHANOL

 

PREPARATION OF CLEANING SOLUTION: ETHANOL

INTRODUCTION

Ethanol is a flammable chemical. If not stored and handled properly, this can pose a serious threat to the health and safety of laboratory personnel, emergency responders and chemical waste handlers. Hence, it is important to follow safety protocols to handle this chemical. Ethanol is mainly used as solvent. It is miscible with water and with many organic solvents, including acetic acid, acetone, benzene, carbon tetrachloride, chloroform, diethyl ether, ethylene glycol, glycerol, nitromethane, pyridine, and toluene. It is also miscible with light aliphatic hydrocarbons, such as pentane and hexane, and with chlorinated organic such as trichloroethane and tetrachloroethylene.

AIM:

To prepare the cleaning solution for disinfect the surface of working table and glassware.

MATERIALS REQUIRED:

            Measuring jar, 0.2 micron filter, Distilled water, 99.9% ethanol, Filtration apparatus, Glass bottle

PROCEDURE:

1.      Assemble the filtration unit to achieve filter sterilization to the water and the 99.9% lab ethanol. All the steps shall be performed in to the LAF to avoid contamination.

2.      Pour the water in to the 0.2 micron filter containing filtration cup and turn on the pumb.

3.      Repeat the procedure for sterilizing the lab ethanol.

4.      To prepare 1 liter of 70% Ethanol using 95% lab Ethanol add 740ml of lab ethanol to 260ml water.

5.      Label the container and store in a safe place.

RESULT:

Ethanol cleaning solution has been prepared as followed the procedure for the disinfection purpose and stored in a secure place. The effectiveness of the cleaning solution will be checked periodically.